Plasmid
Part:BBa_I713025
Designed by: Zhenyu Shi Group: iGEM07_Tsinghua (2007-10-25)
pEB-lambda
pEASY-B blue/white screen vector with designed FRT flaked insert to block the lacZ activity.
When FLP is applied, the FRT insert will be deleted and the vector will recover the lacZ activity.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 381
Illegal XbaI site found at 1185
Illegal XbaI site found at 1290
Illegal SpeI site found at 258
Illegal PstI site found at 403
Illegal PstI site found at 1250
Illegal PstI site found at 2440 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 740
Illegal SpeI site found at 258
Illegal PstI site found at 403
Illegal PstI site found at 1250
Illegal PstI site found at 2440
Illegal NotI site found at 1271 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2227
Illegal BamHI site found at 252
Illegal BamHI site found at 415
Illegal XhoI site found at 1278 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 381
Illegal XbaI site found at 1185
Illegal XbaI site found at 1290
Illegal SpeI site found at 258
Illegal PstI site found at 403
Illegal PstI site found at 1250
Illegal PstI site found at 2440 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 381
Illegal XbaI site found at 1185
Illegal XbaI site found at 1290
Illegal SpeI site found at 258
Illegal PstI site found at 403
Illegal PstI site found at 1250
Illegal PstI site found at 2440
Illegal NgoMIV site found at 1616
Illegal NgoMIV site found at 2891 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 851
Illegal BsaI site found at 3790
Illegal SapI.rc site found at 2740
Illegal SapI.rc site found at 2950
[edit]
Categories
Parameters
resistance | AK |